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Updated: May 18, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
N-glycoproteomics: mass spectrometry-based glycosylation site annotation
Yvonne Pasing1, Albert Sickmann, Urs Lewandrowski
1Department of Bioanalytics, Leibniz-Institut für Analytische Wissenschaften, ISAS eV, D-44227 Dortmund, Germany.
Abstract:
Glycosylations are ubiquitous and, in many cases, essential protein modifications. Yet comprehensive and detailed analysis of glycosylations on a proteome-wide scale is a daunting and still unsolved challenge. However, a common workflow has emerged over the last decade for large-scale N-glycosylation site annotation by application of proteomic methodology. Thereby, the qualitative and quantitative assessment of hundreds or thousands of modification sites is enabled. This review presents a short overview about common enrichment techniques and glycosylation site detection for N-glycopeptides, including benefits and challenges of analysis.
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