Comparing peripheral blood stem cell collection using the COBE Spectra, Haemonetics MCS+, and Baxter Amicus.

Area of Science:

• Hematology
• Transplantation Science
• Biomedical Engineering

Background:

• Peripheral blood stem cells (PBSC) are the primary source for hematopoietic stem cell transplantation.
• Apheresis systems are crucial for collecting these cells for both autologous and allogeneic transplantation.

Purpose of the Study:

• To evaluate and compare the efficacy of three apheresis systems (COBE Spectra, Haemonetics MCS+, Baxter Amicus) for PBSC collection.
• To identify the optimal system for PBSC collection based on product yield and cell counts.

Main Methods:

• A retrospective analysis of 99 COBE Spectra, 81 Haemonetics MCS+, and 38 Baxter Amicus collections was conducted.
• Data on processed blood volume, PBSC yield, white blood cell (WBC) counts, CD34+ cell counts, and platelet collection were analyzed.
• Statistical comparisons were made between the three systems.

Main Results:

• The Baxter Amicus processed larger blood volumes and yielded larger PBSC volumes.
• The COBE Spectra produced significantly more WBC, WBC/liter, and WBC/kg compared to the other systems.
• CD34+ cell yields were comparable between Spectra and MCS+, while Amicus collected fewer platelets.
• A linear correlation was observed between circulating CD34+ cells and total CD34+ cells in products across all systems.

Conclusions:

• The COBE Spectra system is recommended for PBSC collection due to its higher WBC yield while maintaining comparable CD34+ cell counts.
• The Spectra system offers advantages such as an automated procedure, no rotating seal, and small extracorporeal volume.
• The linear correlation supports accurate prediction of CD34+ cell yield based on pre-collection counts.