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Complete cDNA sequence and chromosomal localization of mouse alpha 1-antitrypsin
R N Sifers1, F D Ledley, L Reed-Fourquet
1Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Genomics
|January 1, 1990
Summary
Researchers cloned and sequenced murine alpha 1-antitrypsin (Aat), finding it homologous to human Aat. This suggests conserved functions and locates the Aat gene on mouse chromosome 12.
Area of Science:
- Molecular biology
- Genetics
- Biochemistry
Background:
- Alpha 1-antitrypsin is a key protease inhibitor.
- Understanding its murine homolog provides insights into mammalian protein function.
Purpose of the Study:
- To clone and sequence the murine alpha 1-antitrypsin (Aat) gene.
- To analyze the structural and functional conservation between murine and human Aat.
- To determine the chromosomal localization of the Aat gene in mice.
Main Methods:
- cDNA cloning and sequencing of the open reading frame of murine alpha 1-antitrypsin.
- Bioinformatic analysis of nucleic acid and predicted amino acid sequences.
- In situ hybridization for gene localization.
Main Results:
- The complete open reading frame of murine alpha 1-antitrypsin was successfully cloned and sequenced.
- Sequence homology was observed between murine and human alpha 1-antitrypsin, indicating conserved structural determinants for targeting, glycosylation, and function.
- The alpha 1-antitrypsin gene locus (Aat) was mapped to murine chromosome 12E-F.
Conclusions:
- Murine alpha 1-antitrypsin shares significant homology with its human counterpart, suggesting conserved biological roles.
- The identified gene locus provides a genetic marker for further studies on Aat function and regulation in mice.