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Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

A novel tissue microarray instrumentation: the HT-1 tissue microarrayer.

Jun Yang¹, Mingjuan Zhang, Baoshan Su

¹Department of Pathology, Second Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China.

Indian Journal of Pathology & Microbiology

|October 4, 2012

Summary

The new HT-1 tissue microarrayer offers a simple, economical, and user-friendly solution for generating tissue microarrays (TMAs). This automated device efficiently creates array pores and embeds tissues, improving gene expression analysis.

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Area of Science:

Biomedical Engineering
Histopathology
Molecular Biology

Background:

Tissue microarray (TMA) technology enables efficient analysis of gene expression in histological tissues.
Existing automated tissue arrayers lack cost-efficiency and ease of use.

Purpose of the Study:

To design and produce an automated tissue microarrayer (HT-1) that is cost-efficient and user-friendly.
To improve instrumentation for generating TMAs.

Main Methods:

The HT-1 tissue microarrayer was developed, featuring an array pore instrument, punch needle, negative-pressure embedding system, and manipulator.
Fourteen TMAs were constructed, accommodating 312 tissue cases.
TMA sections were validated using hematoxylin-eosin staining, in situ hybridization, and immunohistochemistry.

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Last Updated: May 18, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

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Published on: September 23, 2014

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Published on: May 31, 2012

Main Results:

Hematoxylin-eosin staining confirmed consistent and ordered tissue cylinders.
The HT-1 rapidly creates array pores in paraffin blocks within seconds.
A unique negative pressure system facilitates consistent embedding and sectioning of tissue cylinders.

Conclusions:

The HT-1 tissue microarrayer is a simple, economical, and easy-to-use device.
This instrument enhances the efficiency and accessibility of TMA generation for research.