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Updated: May 18, 2026

Polytetrafluoroethylene (PTFE) as a Suture Material in Tendon Surgery
Published on: October 6, 2022
In vitro microbiologic evaluation of PTFE and cotton as spacer materials
Avina Paranjpe1, Sumita Jain, Karim J Alibhai
1Department of Endodontics, University of Washington, Seattle, WA, USA. avina@u.washington.edu
Objective:
To microbiologically evaluate the efficacy of cotton and polytetrafluoroethylene (PTFE) tape used as spacer materials.
Method And Materials:
Twenty-six extracted human molars were restored using either cotton or PTFE tape as spacers under a standardized provisional restorative material (Cavit). The teeth were incubated for 7 days in a culture of Streptococcus gordonii or in liquid media alone. The spacers were removed and tested for bacterial contamination. The access cavities were also evaluated for bacterial contamination.
Results:
Nine of 10 teeth with cotton spacers and one of 10 teeth with PTFE spacers were positive for S gordonii growth. The nine teeth in the cotton group also showed contamination of the access cavities.
Conclusion:
Even under optimal conditions, cotton spacers may cause leakage into the access cavities. Cotton fibers may serve as a route for bacterial contamination of the access cavities and root canal space. In contrast, PTFE tape did not provide an avenue for bacterial contamination.
