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Updated: May 18, 2026

Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
HIV-1 antisense transcription is preferentially activated in primary monocyte-derived cells
Sylvain Laverdure1, Antoine Gross, Charlotte Arpin-André
1Université Montpellier 1, Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France.
Abstract:
In this study, an antisense luciferase-expressing human immunodeficiency virus type 1 (HIV-1) molecular clone was used to infect primary cells. We found that antisense transcription activity from the 3' long terminal repeat (LTR) was significantly more abundant in monocyte-derived cells than in activated T lymphocytes. Moreover, by analyzing antisense transcription in infected monocyte-derived dendritic cells (MDDCs), we observed that the majority of HIV-1-infected MDDCs with significant antisense transcription activity did not produce Gag. We also confirmed that the negative-strand-encoded antisense protein (ASP) was expressed in monocyte-derived cells.
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