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Updated: May 18, 2026
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
Published on: November 1, 2013
Effects of flavone derivatives on antigen-stimulated degranulation in RBL-2H3 cells
Doseok Hwang1, Hye-Jin Park, Eun-Kyung Seo
1Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul 143-701, Korea National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea Cell Activation Research Institute, Gyeonggi-do 464-870, Korea.
Abstract:
Mast cells are primarily responsible for IgE-mediated allergic responses. The activation level of mast cells is reflected in their degree of degranulation. This can in turn be determined by measuring the amount of β-hexosaminidase release, a key parameter in degranulation. In this study, 40 flavone derivatives, including flavone, 14 methoxyflavones, 13 hydroxyflavones, and 12 hydroxymethoxyflavones, were evaluated for their inhibition of degranulation in rat basophilic leukemia RBL-2H3 cells. 3',7-Dihydroxyflavone inhibited degranulation (IC(50) = 13.56 μM), which was comparable to PP2 (3-(4-chlorophenyl)-1-(1,1-dimethylethyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine) used as a control. In addition, we report quantitative relationships between the structural properties of flavones and their inhibitory effects on degranulation.
