Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harnessing artificial intelligence in plant breeding: innovations in digital phenotyping and breeding methodologies.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same author

Evolutionary structure constrains genomic prediction accuracy more than model complexity in mango (Mangifera indica L.).

G3 (Bethesda, Md.)·2026
Same author

Strategic global data integration to improve genomic prediction accuracy in tree breeding programs facing resource limitations, a case study in mango.

Horticulture research·2026
Same author

Large scale wheat data integration improves genomic prediction accuracy with the potential to facilitate international breeding collaborations.

Communications biology·2026
Same author

Towards holistic phenotype prediction beyond genotypic data.

Journal of experimental botany·2026
Same author

Genomic exploration of durable wheat rust resistance by integrating data from multiple worldwide populations.

The plant genome·2025

Related Experiment Video

Updated: May 18, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Microsatellites grant more stable flanking genes.

Reem Joukhadar1, Abdulqader Jighly

  • 1University of Aleppo, Aleppo, Syria.

BMC Research Notes
|October 6, 2012
PubMed
Summary

Microsatellites (SSRs) enhance DNA stability by promoting replication by robust DNA polymerases. Flanking genes with SSRs improves gene transfer stability and reduces mutation risks in transformation experiments.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Microsatellites, also known as simple sequence repeats (SSRs), are highly mutable DNA sequences found in all genomes.
  • Previous research indicates that regions flanking SSRs exhibit remarkable stability across diverse organisms.

Purpose of the Study:

  • To investigate the hypothesis that SSRs enhance flanking DNA stability by selectively promoting replication by more robust DNA polymerases.
  • To present evidence for the molecular basis of SSR-mediated DNA stability.

Main Methods:

  • Examining DNA polymerase activity during Polymerase Chain Reactions (PCRs) using SSR loci with varying lengths.
  • Comparing the mutational rate of transferred genes in transformation experiments with naked T-DNA versus T-DNA flanked by SSRs.

More Related Videos

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

Related Experiment Videos

Last Updated: May 18, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

Main Results:

  • SSR sequences facilitate the preferential replication of flanking DNA by stable DNA polymerases, discarding less stable ones.
  • T-DNA fragments flanked by SSRs demonstrate increased stability during gene transfer.

Conclusions:

  • Flanking T-DNA with SSRs in transformation experiments leads to more stable gene transfer.
  • This strategy mitigates risks associated with mutational pressure on foreign DNA segments.