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Published on: June 7, 2019
A switchable two-photon membrane tracer capable of imaging membrane-associated protein tyrosine phosphatase
Lin Li1, Xiaoqin Shen, Qing-Hua Xu
1Department of Chemistry, National University of Singapore, Singapore.
Angewandte Chemie (International Ed. in English)
|October 6, 2012
Abstract:
When I look into your cells: a two-photon dye (Flu7) was developed, which strongly fluoresces only upon selective binding to the plasma membrane of mammalian cells. Upon addition of a UV- and phosphatase-controlled quencher (Q12), the system exhibits ON/OFF/ON fluorescence switching and can be used to image membrane-associated receptor-like protein tyrosine phosphatase (RPTP) activity.

