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Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

V M Corman1, I Eckerle, T Bleicker

  • 1Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.

Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin
|October 9, 2012
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...