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Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
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Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...

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Related Experiment Video

Updated: May 18, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

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Published on: March 1, 2013

Surface modified poly(β amino ester)-containing nanoparticles for plasmid DNA delivery.

Rachel J Fields1, Christopher J Cheng, Elias Quijano

  • 1Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|October 9, 2012
PubMed
Summary

Biodegradable nanoparticles using poly(lactic-co-glycolic acid) and poly(beta-amino) ester offer safe gene delivery. Surface modification with cell-penetrating peptides enhances plasmid DNA loading and transfection efficiency while reducing toxicity.

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Published on: August 13, 2021

Area of Science:

  • Biomaterials science
  • Gene therapy
  • Nanotechnology

Background:

  • Viral and liposomal vectors pose safety concerns for gene therapy.
  • Biodegradable polymers offer a promising alternative for plasmid DNA delivery.
  • Poly(lactic-co-glycolic acid) (PLGA) and poly(beta-amino) esters (PBAE) are key biodegradable polymers.

Purpose of the Study:

  • To formulate and characterize novel nanoparticle (NP) systems for gene delivery using PLGA and PBAE blends.
  • To evaluate the impact of PBAE content on NP properties, cellular uptake, and transfection efficiency.
  • To investigate the efficacy of cell-penetrating peptide (CPP) surface modification in improving NP performance and reducing toxicity.

Main Methods:

  • Formulation of PLGA-PBAE blended nanoparticles at various weight/weight ratios.
  • Characterization of NPs for size, morphology, plasmid DNA (pDNA) loading, and surface charge.
  • Assessment of cellular internalization and transfection efficiency in COS-7 and CFBE41o- cells.
  • Surface modification of NPs with CPPs (mTAT, bPrPp, MPG) using a DSPE-PEG2000 linker.
  • Evaluation of cytotoxicity and pDNA loading/transfection efficiency of CPP-modified NPs.

Main Results:

  • NPs containing PBAE showed enhanced cellular internalization and transfection compared to PLGA-only NPs.
  • PBAE incorporation led to increased cytotoxicity, presenting an engineering challenge.
  • CPP surface coating significantly reduced NP surface charge and cellular toxicity.
  • CPP-modified NPs exhibited improved pDNA loading, intracellular uptake, and transfection efficiency.
  • bPrPp and MPG coatings resulted in 3-4.5x higher pDNA loading and an order of magnitude increase in transfection efficiency.

Conclusions:

  • Surface-modified PBAE-containing NPs represent a highly effective and minimally toxic platform for plasmid DNA delivery.
  • CPP functionalization is a viable strategy to overcome the limitations of PBAE-based gene delivery systems.
  • These findings advance the development of safe and efficient non-viral gene delivery vectors for therapeutic applications.