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Detection of RD-114 virus by a reverse transcriptase assay based on product enhancement

Rie Narushima1, Naoya Tamura, Masayuki Iida

  • 1National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan. narusima@nval.maff.go.jp

Biologicals : Journal of the International Association of Biological Standardization
|October 9, 2012
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...