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Related Experiment Video

Updated: May 18, 2026

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

Published on: February 24, 2011

Exercise intensity and lymphocyte subset apoptosis.

J W Navalta¹, S Lyons, J Prestes

¹Kinesiology and Nutrition Sciences, University of Nevada-Las Vegas, Las Vegas, NV, USA. james.navalta@unlv.edu

International Journal of Sports Medicine

|October 9, 2012

Summary

Exercise intensity impacts immune cell responses. Moderate exercise increases apoptosis in CD4+ and CD8+ T-cells, while high intensity promotes cell migration, indicating shifts in immune cell behavior during exertion.

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Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

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Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

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Published on: January 29, 2019

Area of Science:

Immunology
Exercise Physiology
Cell Biology

Background:

Understanding how immune cell subsets respond to varying exercise intensities is crucial for comprehending exercise-induced immune modulation.
Lymphocyte subsets, including CD4+, CD8+, and CD19+ cells, play vital roles in immune function and may be affected by physical stress.

Purpose of the Study:

To investigate the differential responses of lymphocyte subsets (CD4+, CD8+, CD19+) to increasing exercise intensities.
To assess changes in cell concentration, apoptosis (via Annexin V staining), and cell migration (via CX₃CR1 expression) in response to graded exercise.

Main Methods:

Participants underwent a maximal oxygen uptake (VO2max) test followed by exercise bouts at 76%, 87%, and 100% VO2max.
Blood samples were collected at rest, post-exercise intensity, and 1-hour post-exercise.
Flow cytometry was used to analyze cell concentration, Annexin V binding (apoptosis marker), and CX₃CR1 expression (migration marker) on lymphocyte subsets.

Main Results:

Absolute increases in CD4+/Annexin V+ and CD8+/Annexin V+ cells were observed at 76% VO2max, suggesting apoptosis induction.
Significant absolute increases in CD4+/CX₃CR1 at 87% VO2max and CD8+/CX₃CR1 at 87% and 100% VO2max indicated enhanced cell migration.
Relative changes in CX₃CR1 expression were noted 1-hour post-exercise for CD8+ and CD19+ subsets, while no relative changes in apoptosis markers were found.

Conclusions:

Lymphocyte subsets exhibit distinct responses to exercise intensity concerning cell count, apoptosis, and migration.
Moderate exercise (76% VO2max) appears to induce apoptosis in CD4+ and CD8+ T-cells.
Higher exercise intensities (≥87% VO2max) promote significant migration of CD4+ and CD8+ T-cells from the vasculature, as evidenced by increased CX₃CR1 expression.