Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases
Megan Wheeler1, Antoine Chardon, Astrid Goubet
1School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane 4072, Australia.
Abstract:
Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.
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