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Related Experiment Videos

Mouse strain differences in resident peritoneal cells: a flow cytometric analysis.

M F Festing1, R Legg, T Eydmann

  • 1Medical Research Council Toxicology Unit, Carshalton, Surrey, UK.

Laboratory Animals
|January 1, 1990
PubMed
Summary

Mouse strain significantly impacts peritoneal cell composition and function. Macrophage-to-lymphocyte ratios and phagocytosis vary widely, necessitating consideration in experimental design using resident peritoneal cells.

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Area of Science:

  • Immunology
  • Cell Biology
  • Genetics

Background:

  • Resident peritoneal cells play a crucial role in immune surveillance and response within the peritoneal cavity.
  • Variability in immune cell populations between mouse strains can significantly influence experimental outcomes.
  • Understanding baseline cellular differences is essential for reproducible immunological research.

Purpose of the Study:

  • To quantify and compare the cellular composition and phagocytic capacity of resident peritoneal cells across eight different mouse strains.
  • To identify significant inter-strain variations in key immune cell populations and their functional parameters.
  • To provide data that aids researchers in selecting appropriate mouse strains for studies involving resident peritoneal cells.

Main Methods:

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  • Flow cytometry was employed to analyze the cellular populations, specifically focusing on the ratio of macrophages to macrophages plus lymphocytes.
  • Phagocytosis assays using fluorescent beads were conducted to assess the uptake capacity of peritoneal cells.
  • Quantitative analysis of total peritoneal cells and macrophage yield per mouse was performed for each strain.

    • Main Results:

    • A more than twofold variation in the macrophage to (macrophages + lymphocytes) ratio was observed, ranging from 27% in A/J to 62% in C57B/L10 mice.
    • Significant strain-specific differences were noted in other cellular parameters, including a notable lymphocyte deficiency in CBA/N mice (carrying the xid mutation).
    • Phagocytic activity varied, with bead uptake ranging from 0.99 cells/cell in MFI to 1.64 cells/cell in BALB/c mice over 20 minutes. Total peritoneal cell counts and macrophage yields also showed considerable inter-strain variability.

    Conclusions:

    • Significant genetic variation exists in the resident peritoneal cell populations and their functions among different mouse strains.
    • These inherent differences, particularly in cell ratios and phagocytic capacity, must be carefully considered when designing experiments.
    • Appropriate strain selection is critical for the validity and reproducibility of research utilizing resident peritoneal cells.