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Effect of anti-adhesion barrier solution containing ciprofloxacin-hydrocortisone on abraded mucosa with otitis media
Chul Ho Jang1, Yong Beom Cho, Cheol Hee Choi
1Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea. chulsavio@hanmail.net
Objective:
No study to date has assessed the anti-adhesive effect of new middle ear (ME) packing agents in. This study compared the anti-inflammatory and anti-adhesive effect of antibiotic-steroid containing packing agents in abraded mucosa of the ME inflammation.
Materials And Methods:
Transbullar injection of a saline suspension of Pseudomonas aeruginosa lipopolysaccharide (LPS) induced otitis media. ME mucosa of guinea pigs was abraded using a pick 30 min after LPS inoculation. The animals were divided into four groups of 10 guinea pigs each. In group A, ME cavity was preserved without any packing. In group B, ME cavity was packed with soluble hyaluronic acid-carboxymethyl cellulose (HA-CMC). In group C, the ME cavity was packed with soluble HA-CMC. In group D, the ME cavity was packed with antibiotic-steroid containing soluble HA-CMC. Otoendoscopic examination, auditory brainstem responses (ABRs), and radiographic examination using computerized tomography (CT) were performed at 2 weeks post-surgery. Histopathological evaluation for ME mucosa was performed by light microscopy (LM) and scanning electron microscopy (SEM).
Results:
Otoendoscopic findings and CT findings revealed that group D showed the best recovery of aeration in the ME compared to other groups. Recovery of ABRs threshold was significantly attenuated in group D. In LM and SEM findings, group D showed normalized mucosal thickening compared to other groups.
Conclusion:
ME packing by antibiotic-steroid containing soluble HA-CMC may be useful in the abraded mucosa of the ME inflammation.
Insights
New middle ear (ME) packing agents containing antibiotics and steroids significantly improved healing in inflamed, abraded ME mucosa. This packing enhanced aeration and reduced mucosal thickening, suggesting its potential clinical utility.
Area of Science:
- Otolaryngology
- Biomaterials Science
- Inflammation Research
Background:
- Middle ear (ME) inflammation and mucosal abrasion present challenges in otologic surgery.
- The anti-adhesive and anti-inflammatory effects of novel ME packing agents require investigation.
Purpose of the Study:
- To evaluate the anti-inflammatory and anti-adhesive properties of antibiotic-steroid containing packing agents in an abraded ME mucosa model.
- To compare the efficacy of different ME packing materials, including hyaluronic acid-carboxymethyl cellulose (HA-CMC) and HA-CMC with antibiotic-steroid.
Main Methods:
- Otitis media was induced in guinea pigs using Pseudomonas aeruginosa lipopolysaccharide (LPS).
- ME mucosa was abraded, and animals were assigned to four groups: no packing, HA-CMC packing, or antibiotic-steroid containing HA-CMC packing.
- Otoendoscopy, auditory brainstem responses (ABRs), CT scans, light microscopy (LM), and scanning electron microscopy (SEM) were used for evaluation.
Main Results:
- The antibiotic-steroid containing HA-CMC packing group demonstrated superior recovery of ME aeration and ABR thresholds.
- Histopathological analysis revealed normalized mucosal thickening in the group treated with antibiotic-steroid containing HA-CMC.
- CT and otoendoscopic findings supported improved healing in the antibiotic-steroid packing group.
Conclusions:
- Middle ear packing with antibiotic-steroid containing soluble HA-CMC shows promise for treating inflamed and abraded ME mucosa.
- This novel packing agent may offer significant benefits in promoting healing and restoring function in specific otologic conditions.
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