Education: DNA replication using microscale natural convection

Area of Science:

• Interdisciplinary science education
• Chemical engineering
• Molecular biology
• Microfluidics

Background:

• Need for educational experiences unifying physical, chemical, and life sciences.
• Microfluidics as a versatile tool for interdisciplinary learning.
• Importance of applying knowledge to societal benefit through new technologies.

Purpose of the Study:

• Introduce chemical engineering students to molecular biology.
• Develop hands-on activities using microscale natural convection for DNA replication (polymerase chain reaction - PCR).
• Investigate the positive impact of these activities on student learning.

Main Methods:

• Construction of convective PCR stations with microfluidic reactors.
• Utilizing a motion analysis microscope for visualizing flow patterns with fluorescent tracers.
• Developing computational fluid dynamics (CFD) exercises to model microscale thermal convection.
• Conducting cognitive assessments to evaluate learning outcomes.

Main Results:

• Successful implementation of hands-on activities integrating microfluidics and molecular biology.
• Demonstration of microscale natural convection for polymerase chain reaction (PCR).
• Positive impact on student learning identified through cognitive assessments.

Conclusions:

• Microfluidic-based hands-on activities effectively teach fundamental principles at the physical, chemical, and life science interface.
• Harnessing microscale natural convection for DNA replication is a viable educational strategy.
• Interdisciplinary learning experiences positively influence student comprehension and engagement.