A highly sensitive and temporal visualization system for gaseous ethanol with chemiluminescence enhancer

Area of Science:

• Analytical Chemistry
• Biochemistry

Background:

• Developing sensitive methods for gaseous ethanol detection is crucial for environmental monitoring and industrial safety.
• Existing visualization techniques may lack real-time spatial and temporal resolution.

Purpose of the Study:

• To develop and demonstrate a two-dimensional gaseous ethanol visualization system.
• To enhance chemiluminescence (CL) intensity for improved detection limits.

Main Methods:

• Utilized a horseradish peroxidase-luminol-hydrogen peroxide system with an enzyme-immobilized membrane.
• Employed high-purity luminol sodium salt (L-HG) and eosin Y (EY) as a CL enhancer.
• Measured ethanol concentration via CL intensity recorded by a CCD camera.

Main Results:

• Achieved linear correlation between CL intensity and gaseous ethanol concentration.
• Demonstrated real-time spatial and temporal analysis capabilities.
• Established a detection limit of 3 ppm for gaseous ethanol with optimized L-HG and EY concentrations.

Conclusions:

• The developed two-dimensional CL system provides an effective method for visualizing gaseous ethanol.
• The system offers high sensitivity and real-time analytical capabilities for gas component monitoring.
• Optimization with L-HG and EY significantly improved CL intensity and detection performance.