Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 17, 2026

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
05:27

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Published on: July 20, 2022

Complex I assay in mitochondrial preparations from CNS.

Latha Diwakar1, Ajit Ray, Vijayalakshmi Ravindranath

  • 1National Brain Research Centre, Nainwal Mode, Manesar, India.

Current Protocols in Toxicology
|October 10, 2012
PubMed
Summary

This study presents a simple spectrophotometric method to measure complex I activity in mouse brain mitochondria. This technique aids research into neurodegenerative disorders linked to complex I dysfunction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole-genome sequencing of 197 cases with Parkinson's disease reveals novel pathogenic variants in the Indian population.

NPJ Parkinson's disease·2026
Same author

Correction: Gulf war toxicant-induced effects on the hippocampal dendritic arbor are reversed by treatment with a <i>Withania somnifera</i> extract.

Frontiers in neuroscience·2025
Same author

Correction: Rural-urban differences in lipid abnormalities among middle‑aged and older Indians.

BMC public health·2025
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Public Health.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

SIRT2 attenuates stress-induced skeletal muscle atrophy by inhibiting glucocorticoid receptor signalling.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Biochemistry
  • Neuroscience
  • Mitochondrial Biology

Background:

  • Mammalian NADH:ubiquinone oxidoreductase (complex I) is a large enzyme complex crucial for mitochondrial respiration.
  • Complex I dysfunction is implicated in neurodegenerative diseases and is sensitive to reactive oxygen species.
  • Assessing complex I activity is vital for understanding its role in health and disease.

Purpose of the Study:

  • To develop a straightforward spectrophotometric assay for quantifying complex I activity.
  • To enable the study of complex I function in specific brain regions of mice.
  • To provide a tool for investigating the link between complex I and neurodegeneration.

Main Methods:

  • Isolation of mitochondria from specific regions of the mouse central nervous system.

More Related Videos

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
15:04

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Published on: September 28, 2019

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
05:45

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples

Published on: May 3, 2024

Related Experiment Videos

Last Updated: May 17, 2026

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
05:27

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Published on: July 20, 2022

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
15:04

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Published on: September 28, 2019

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
05:45

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples

Published on: May 3, 2024

  • Spectrophotometric measurement of NADH:ubiquinone oxidoreductase activity.
  • Standard biochemical assay techniques.

    • Main Results:

    • A simple and effective spectrophotometric method for estimating complex I activity was established.
    • The method allows for the analysis of complex I from isolated brain mitochondria.
    • This provides a valuable tool for future research into complex I function.

    Conclusions:

    • The developed spectrophotometric method is suitable for assessing complex I activity in mouse brain mitochondria.
    • This assay facilitates research into the role of complex I dysfunction in neurodegenerative disorders.
    • The technique offers a simple approach to study a key component of the electron transport chain.