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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

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Related Experiment Video

Updated: May 17, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants
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In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

In situ hybridization histochemistry.

Z Kokaia1

  • 1University Hospital, Lund, Sweden.

Current Protocols in Toxicology
|October 10, 2012
PubMed
Summary

This study details two in situ hybridization methods using radiolabeled or digoxigenin-labeled probes for detecting messenger RNA (mRNA) distribution and expression in frozen tissue sections. Protocols cover probe labeling and sample preparation for accurate molecular analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • In situ hybridization (ISH) is crucial for visualizing nucleic acid targets within biological tissues.
  • Accurate detection of messenger RNA (mRNA) expression requires robust and reliable methodologies.
  • Standard ISH protocols often necessitate optimization for specific probe types and sample preparations.

Purpose of the Study:

  • To describe two distinct in situ hybridization techniques for mRNA detection.
  • To provide detailed protocols for probe labeling and sample preparation.
  • To enable the accurate assessment of mRNA physical distribution and expression levels.

Main Methods:

  • In situ hybridization using an ³⁵S-labeled oligonucleotide probe on cryostat-sectioned samples.

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A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
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A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

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Last Updated: May 17, 2026

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A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
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A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

Published on: August 13, 2009

  • In situ hybridization using a digoxigenin-labeled oligonucleotide probe on cryostat-sectioned samples.
  • Development of protocols for probe labeling and tissue sample preparation.

    • Main Results:

    • Successful implementation of two distinct ISH methods for mRNA detection.
    • Demonstration of the ability to determine the physical location of target mRNA.
    • Quantification of target mRNA expression levels within tissue sections.

    Conclusions:

    • The described in situ hybridization methods are effective for analyzing mRNA distribution and expression.
    • The provided protocols facilitate reproducible molecular analysis in frozen tissue samples.
    • These techniques are valuable for research in developmental biology, neuroscience, and disease pathology.