Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 17, 2026

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
12:32

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

Published on: November 12, 2011

Coculturing neurons and glial cells.

Barbara Viviani1

  • 1University of Milan Via Balzaretti, Milan, Italy.

Current Protocols in Toxicology
|October 10, 2012
PubMed
Summary

Neurotoxic effects involve neuron-glia interactions. Coculture models allow studying how glial cells, including astrocytes and microglia, propagate neurodegeneration after neurotoxicant exposure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developmental neurotoxicity of atrazine: decreased synaptic spine density and miRNA-driven GluN2A downregulation in primary hippocampal neurons.

Archives of toxicology·2026
Same author

Statement on the findings presented in the Toxicological Profile for Mercury issued by the US ATSDR.

EFSA journal. European Food Safety Authority·2026
Same author

Methods2AOP: A Collaboration to Strengthen the Integration of Test Methods into the Adverse Outcome Pathway Framework.

F1000Research·2026
Same author

Transcript-protein discrepancy of glutamatergic receptor subunits in human iPSC-derived neurons: Implications for neurotoxicity testing.

Toxicology letters·2026
Same author

Derivation of a health-based guidance value for Δ<sup>8</sup>-tetrahydrocannabinol (Δ<sup>8</sup>-THC) and its occurrence in food.

EFSA journal. European Food Safety Authority·2025
Same author

17-α-ethinyl estradiol in vitro treatment of rat primary hippocampal neurons shapes glutamatergic developmental program: molecular and morphological dynamics at the postsynapse.

European journal of pharmacology·2025

Area of Science:

  • Neuroscience
  • Cell Biology
  • Toxicology

Background:

  • Neurons, glia, astroglia, and astrocytes are crucial in brain neurotoxicity.
  • Glial cells activate upon neurotoxicant exposure, releasing damaging substances.
  • Neural death and glia-glia communication mediate damage propagation.

Purpose of the Study:

  • To describe protocols for neuron-glia and microglia-astrocyte sandwich cocultures.
  • To investigate complex neuron-glia interactions in neurodegeneration.
  • To provide a model for studying glial cell roles in neurotoxicity.

Main Methods:

  • Setting up neuron-glia sandwich cocultures.
  • Establishing microglia-astrocyte sandwich cocultures.
  • Utilizing separated cell populations on different surfaces for analysis.

Main Results:

  • Developed controlled coculture systems for studying neuron-glia interactions.
  • Enabled separation of cell populations without compromising integrity.
  • Facilitated investigation of mediator release and damage propagation.

Conclusions:

  • Sandwich cocultures are valuable tools for investigating neuron-glia relationships in neurodegeneration.
  • This model allows detailed study of glial cell activation and communication.
  • The system supports research into neurotoxic mechanisms and potential interventions.

More Related Videos

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
11:08

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Published on: November 14, 2016

Related Experiment Videos

Last Updated: May 17, 2026

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
12:32

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

Published on: November 12, 2011

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
11:08

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Published on: November 14, 2016