Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Halothane inhibits 5-hydroxytryptamine uptake by synaptosomes from rat brain.

D C Martin1, R J Adams, R P Introna

  • 1Department of Anesthesiology and Physiology and Endocrinology, Medical College of Georgia, Augusta 30912.

Neuropharmacology
|January 1, 1990
PubMed
Summary

General anesthetics like halothane disrupt serotonin (5-HT) transport in the brain. This study shows halothane competitively inhibits 5-HT uptake in rat brain synaptosomes, suggesting a mechanism for anesthetic action.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The severity and frequency distribution of endometriosis subtypes at different ages: a model to understand the natural history of endometriosis based on single centre/single surgeon data.

Facts, views & vision in ObGyn·2021
Same author

Corrigendum. Nonvisualized palpable bowel endometriotic satellites.

Human reproduction (Oxford, England)·2021
Same author

Nonvisualized palpable bowel endometriotic satellites.

Human reproduction (Oxford, England)·2021
Same author

Correction: Heterogeneity of endometriosis lesions requires individualisation of diagnosis and treatment and a different approach to research and evidence based medicine.

Facts, views & vision in ObGyn·2020
Same author

Infection as a potential cofactor in the genetic-epigenetic pathophysiology of endometriosis: a systematic review.

Facts, views & vision in ObGyn·2020
Same author

Heterogeneity of endometriosis lesions requires individualisation of diagnosis and treatment and a different approach to research and evidence based medicine.

Facts, views & vision in ObGyn·2019

Area of Science:

  • Neuroscience
  • Pharmacology
  • Anesthesiology

Background:

  • Serotonin (5-hydroxytryptamine; 5-HT) plays a key role in central nervous system functions, including sleep and pain perception.
  • General anesthetics may influence 5-HT homeostasis, but their precise mechanisms of action are not fully understood.
  • Investigating the interaction between anesthetics and neurotransmitter systems is crucial for understanding anesthetic effects.

Purpose of the Study:

  • To investigate the effect of the anesthetic halothane on the uptake of serotonin (5-HT) in rat brain synaptosomes.
  • To determine if halothane alters 5-HT homeostasis by affecting its transport.
  • To identify potential sites of anesthetic action within the central nervous system.

Main Methods:

  • Preparation of synaptosomal fractions from rat brain using established techniques.

Related Experiment Videos

  • Measurement of high-affinity transport of radiolabeled 5-HT into synaptosomes.
  • Assessment of halothane's concentration-dependent effect on 5-HT uptake and release.
  • Kinetic analysis (Lineweaver-Burk) to determine the type of inhibition.

    • Main Results:

    • Halothane inhibited the accumulation of 5-HT in synaptosomes in a concentration-dependent manner.
    • The inhibition of 5-HT uptake was significant, with 43% inhibition at 1 mM and 75% at 5 mM halothane.
    • Halothane showed minimal effect on the passive or spontaneous release of accumulated 5-HT.
    • Kinetic analysis indicated that halothane competitively inhibits 5-HT uptake.

    Conclusions:

    • Halothane interferes with the high-affinity uptake of serotonin (5-HT) in the central nervous system.
    • This competitive inhibition of 5-HT transport represents a potential mechanism for the action of halothane as a general anesthetic.
    • The findings suggest that modulation of serotonin transport could be a key target for anesthetic drugs.