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[Effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells]
Dan Li1, Qi-shan Zhang, Yang Chu
1School of Pharmaceutical Sciences, China Medical University, Shenyang, China. danlicmu@hotmail.com
Aim:
To investigate the effect of overexpressing rat aldo-keto reductases (AKR7A1) in V79-4 cells on crotonaldehyde-induced mutagenicity.
Methods:
The expression level and enzyme activity of AKR7A1 protein expressed in V79-4 cells were measured by Western blotting and AKR enzyme assay, respectively. HGPRT assay was applied to evaluate the effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells.
Results:
Western blotting confirmed a high expression level of AKR7A1 protein in V79-4 cells. AKR enzyme assay showed that the overexpressed AKR7A1 protein was functionally active. HGPRT assay demonstrated that cells overexpressing AKR7A1 exhibited a significant increase in resistance to crotonaldehyde-induced mutagenicity compared to control cells.
Conclusion:
Overexpression of AKR7A1 can protect V79-4 cells against crotonaldehyde-induced mutagenicity.
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