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Related Experiment Video

Updated: May 17, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Multiple pathways regulate minisatellite stability during stationary phase in yeast.

Maire K Kelly¹, Laura Brosnan, Peter A Jauert

¹Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.

G3 (Bethesda, Md.)

|October 11, 2012

Summary

This summary is machine-generated.

Genetic factors influencing minisatellite stability were explored. Mutations in END3, PKC1, RAD27, ZRT1, and ZAP1 increase instability, suggesting multiple pathways regulate minisatellite DNA.

Keywords:

DNA stability G0 quiescence stationary phase

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Area of Science:

Genetics
Molecular Biology
Cancer Research

Background:

Minisatellite DNA alterations are linked to human diseases like cancer.
Genetic factors controlling minisatellite stability are not fully understood.
Previous work identified yeast zinc homeostasis genes (ZRT1, ZAP1) affecting minisatellite stability.

Purpose of the Study:

Identify new genes regulating minisatellite stability in yeast.
Investigate the pathways controlling minisatellite stability during stationary phase.
Determine if these pathways affect human minisatellite stability.

Main Methods:

Yeast genetics and mutant screening.
Analysis of minisatellite alteration frequency.
Homologous recombination assays.
Investigation of single-stranded DNA (ssDNA) formation.
Testing human minisatellite tract stability in yeast.

Main Results:

Mutants in END3, PKC1, and RAD27 increase minisatellite instability during stationary phase.
ZRT1, ZAP1, END3, PKC1, and RAD27 function in multiple pathways regulating stability.
Minisatellite alterations occur via homologous recombination.
ssDNA or ssDNA breaks may drive stationary phase instability.
RAD27 loss destabilizes the HRAS1 minisatellite, but END3 and PKC1 do not.

Conclusions:

Multiple genetic pathways, including END3, PKC1, RAD27, ZRT1, and ZAP1, regulate yeast minisatellite stability during stationary phase.
Homologous recombination and potential ssDNA formation are key mechanisms.
Minisatellite stability control is sequence-dependent, as shown by differential effects on the HRAS1 tract.