Related Experiment Video
Updated: May 17, 2026
Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
Published on: January 31, 2025
Detection of Salmonella Enteritidis (SE) bacterin in environmental samples from poultry houses using the RapidChek SE
Rocio Crespo1, Dennis Schaberg, Claire Warren
1Animal Health and Food Safety Laboratory, Washington Animal Disease Diagnostic Laboratory, Washington State University, 2607 W. Pioneer, Puyallup, WA 98371, USA. crespor@wsu.edu
Abstract:
Environmental swabs from pullet houses were tested for the presence of Salmonella Enteritidis (SE) using the RapidChek SE immunoassay test. Of the 1162 samples tested in 2011, 20 samples were positive, but only two samples were confirmed positive for SE by culture. Seventeen positive samples were from pullet houses that had been vaccinated with SE bacterin 2 to 3 days prior to submission to the lab. This study investigated the detection of SE bacterin using the RapidChek SE test system. Swabs were inoculated with different amounts of a commercial SE bacterin. As little as 0.25 ml of the SE bacterin was sufficient to cause positive results with the RapidChek SE test. This finding emphasizes the need to perform bacterial isolation and identification when a positive result occurs using an immunoassay test.
