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Related Experiment Video

Updated: May 17, 2026

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
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Published on: April 12, 2018

Putting vital stains in context.

Nathan Efron1

  • 1Institute of Health and Biomedical Innovation, and School of Optometry and Vision Science, Queensland University of Technology, Kelvin Grove, Queensland, Australia. n.efron@qut.edu.au

Clinical & Experimental Optometry
|October 12, 2012
PubMed
Summary
This summary is machine-generated.

Solution-induced corneal staining (SICS) may be a benign phenomenon, preservative-associated transient hyperfluorescence (PATH), not true corneal staining or lens/solution incompatibility. Differentiating SICS/PATH from pathological staining is key for contact lens wearers.

Keywords:
corneafluoresceinpreservative-associated transient hyperfluorescencesolution-induced corneal stainingvital stains

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Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
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Published on: May 13, 2014

Area of Science:

  • Ophthalmology
  • Corneal Physiology
  • Contact Lens Technology

Background:

  • Vital staining is crucial for diagnosing ocular disease and contact lens issues.
  • Misconceptions exist regarding ophthalmic dyes like sodium fluorescein, rose Bengal, and lissamine green B.
  • Accurate diagnosis requires patient history and assessment of signs/symptoms alongside staining.

Purpose of the Study:

  • To clarify the properties and diagnostic utility of vital stains for ocular surface assessment.
  • To review the etiology of corneal staining, particularly solution-induced corneal staining (SICS).
  • To differentiate SICS/preservative-associated transient hyperfluorescence (PATH) from pathological corneal staining.

Main Methods:

  • Review of existing literature on vital stains and corneal staining.
  • Analysis of the characteristics of SICS/PATH.
  • Comparison of SICS/PATH with pathological corneal staining patterns.

Main Results:

  • Solution-induced corneal staining (SICS) may be a benign phenomenon, preservative-associated transient hyperfluorescence (PATH).
  • SICS/PATH may not indicate true corneal staining or lens/solution incompatibility.
  • SICS/PATH exhibits distinct properties (appearance, duration) differentiating it from pathological staining.

Conclusions:

  • Vital stains have limitations; diagnosis requires a holistic approach.
  • SICS/PATH is likely a benign finding in contact lens wearers, not indicative of pathology.
  • Understanding SICS/PATH is crucial for appropriate patient management and counseling.