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Laser Microdissection for Species-Agnostic Single-Tissue Applications
Published on: March 31, 2022
Laser cutting eliminates nucleic acid cross-contamination in dried-blood-spot processing
Sean C Murphy1, Glenda Daza, Ming Chang
1Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington, USA. murphysc@uw.edu
Journal of Clinical Microbiology
|October 12, 2012
Abstract:
Dried blood spots (DBS) are useful for molecular assays but are prone to false positives from cross-contamination. In our malaria DBS assay, cross-contamination was encountered despite cleaning techniques suitable for HIV-1. We therefore developed a contact-free laser cutting system that effectively eliminated cross-contamination during DBS processing.

