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Allele-specific PCR for determination of IL28B genotype
Linda Cook1, Kurt Diem, Woo Kim
1Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
Journal of Clinical Microbiology
|October 12, 2012
Abstract:
The IL28B genotype is a critical determinant of interferon response in patients infected with hepatitis C virus genotype 1. We describe an allele-specific PCR assay for the IL28B genotype. The assay is simple and robust, uses commonly available real-time PCR instrumentation, and is well suited for clinical laboratories offering IL28B genotyping.
