Related Experiment Video
Updated: May 17, 2026

Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
High cell density cultivation of Pseudomonas putida strain HKT554 and its application for optically active sulfoxide
Said Hamad Ramadhan1, Toru Matsui, Kazuma Nakano
1Tropical Biosphere Research Center, Center of Molecular Biosciences, University of the Ryukyus, 1 Sembaru Nishihara-cho, Okinawa 903-0213, Japan.
Abstract:
Culture conditions with Pseudomonas putida strain HKT554, expressing naphthalene dioxygenase, known as the biocatalyst showing wide substrate specificity, were optimized for high cell density cultivation (HCDC). Culture in a medium TK-B modified from that for HCDC of Escherichia coli with glucose fed-batch and dissolved oxygen stat resulted in a high cell density growth of 114 g dry cell/l at 40 h of cultivation. This system was further applied for S-(+)-methyl phenyl sulfoxide (MPSO) production from methyl phenyl sulfide. Addition of nonpolar organic solvent, such as n-hexadecane, greatly enhanced the MPSO production due to the prevention of substrate evaporation, resulting in a MPSO production up to 39 mM in 30 h with a conversion rate of 95.7 mol%.
More Related Videos
06:24Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
08:58Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
Published on: October 17, 2025
Related Concept Videos
Upstream Processing
Production of Pharmaceuticals
Production of Antibiotics