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Updated: May 17, 2026
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Optimal use of MRSASelect and PCR to maximize sensitivity and specificity of MRSA detection
Mohammad R Hasan1, John D Brunstein, Ghada Al-Rawahi
1Division of Microbiology, Virology and Infection Control, Department of Pathology, Children's and Women's Health Centre of BC, Vancouver, Canada. rubayet.hasan@cw.bc.ca
Abstract:
Suspected colonies of methicillin-resistant Staphylococcus aureus (MRSA) on chromogenic, MRSASelect (BioRad) medium were confirmed using routine microbiological methods, and a multiplex real-time PCR (n = 108). Although the specificity of MRSASelect assessed at 24 h of incubation was much higher than that of 48 h (91.4 vs. 60 %), extending the incubation time to 48 h, along with PCR confirmation, increased the total number of true positive samples by 27.8 %. These results provide a cost effective method for sensitive and specific detection of MRSA.
