Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Karyotyping01:17

Karyotyping

Overview
Karyotyping01:17

Karyotyping

Overview
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of polymorphisms in FSHR gene on late follicular phase progesterone and estradiol serum levels in predicted normoresponders.

Human reproduction (Oxford, England)·2022
Same author

The effect of polymorphisms in FSHR and FSHB genes on ovarian response: a prospective multicenter multinational study in Europe and Asia.

Human reproduction (Oxford, England)·2021
Same author

Sustained intrinsic WNT and BMP4 activation impairs hESC differentiation to definitive endoderm and drives the cells towards extra-embryonic mesoderm.

Scientific reports·2021
Same author

Recent developments in genetics and medically-assisted reproduction: from research to clinical applications<sup>†‡</sup>.

Human reproduction open·2019
Same author

Gain of 20q11.21 in Human Pluripotent Stem Cells Impairs TGF-β-Dependent Neuroectodermal Commitment.

Stem cell reports·2019
Same author

Expression of adhesion and extracellular matrix genes in human blastocysts upon attachment in a 2D co-culture system.

Molecular human reproduction·2018

Related Experiment Video

Updated: May 17, 2026

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Microarray analysis reveals abnormal chromosomal complements in over 70% of 14 normally developing human embryos.

A Mertzanidou1, L Wilton, J Cheng

  • 1Faculty of Medicine and Pharmacy, Research Group Reproduction & Genetics, Vrije Universiteit Brussel, 1090 Brussels, Belgium.

Human Reproduction (Oxford, England)
|October 12, 2012
PubMed
Summary

High rates of chromosomal abnormalities, including mosaicism, are common even in good-quality human embryos selected for in vitro fertilization (IVF). These findings suggest that chromosomal abnormalities are prevalent in early human development, impacting fertility outcomes.

More Related Videos

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
12:32

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

Related Experiment Videos

Last Updated: May 17, 2026

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
12:32

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

Area of Science:

  • Reproductive biology
  • Genetics
  • Embryology

Background:

  • Human fertility rates are low, with in vitro fertilization (IVF) success rates also being relatively low.
  • Chromosomal abnormalities in human gametes and early preimplantation embryos are known to reduce developmental potential.

Purpose of the Study:

  • To determine the rates of aneuploidy and mosaicism in human preimplantation embryos of good quality.

Main Methods:

  • Analysis of 14 good-quality human embryos from young IVF patients (age <35 years) that resulted in healthy births.
  • Blastomeres were collected after thawing cryopreserved Day 2 embryos, cultured, and disaggregated.
  • Array-comparative genomic hybridization (aCGH) was performed on amplified DNA from 91 single blastomeres to detect aneuploidy and structural aberrations.

Main Results:

  • High rates of aneuploidy were observed in good-quality embryos, with 71.4% exhibiting mosaicism.
  • Of the 70 analyzed blastomeres, 44.3% had chromosomal abnormalities, including 29% with structural aberrations.
  • Only 28.6% of the embryos were uniformly diploid; none had the same aneuploidy pattern in all cells.

Conclusions:

  • High-level mosaicism and structural aberrations are prevalent in good-quality IVF embryos, not just those with poor development.
  • These findings necessitate further research into the origins and implications of chromosomal abnormalities in human preimplantation development.