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¹H, ¹³C and ¹⁵N resonance assignments of the complement control protein modules of the complement component C7

Carla Clark1, Chuong-Thu Thai, Marie M Phelan

  • 1Edinburgh Biomolecular NMR Unit, Joseph Black Building, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, Scotland, UK.

Biomolecular NMR Assignments
|October 12, 2012
PubMed

Insights

Researchers determined the structure of human complement C7 complement control protein modules (CCPs). These findings provide insights into the assembly of the membrane attack complex (MAC) and its role in the complement system.

Area of Science:

  • Biochemistry
  • Immunology
  • Structural Biology

Background:

  • Human complement protein C7 is crucial for forming the membrane attack complex (MAC).
  • C7 contains complement control protein (CCP) modules and Factor-I like (FIM) modules.
  • The CCP modules are hypothesized to facilitate C7's binding to C5b.

Purpose of the Study:

  • To elucidate the structure of the C7-CCPs.
  • To provide NMR chemical shift assignments for the C7-CCPs.

Main Methods:

  • Production of a 14-kDa recombinant C7-CCPs protein.
  • Utilized triple-resonance Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Collected backbone and side-chain NMR chemical shift assignments.

Main Results:

  • Achieved 98% backbone and 94% side-chain NMR assignments for C7-CCPs.
  • NMR chemical shifts and assignments were deposited in the BioMagResBank (accession 18530).

Conclusions:

  • The study provides foundational structural data for the C7-CCPs.
  • These assignments are critical for understanding C7's role in MAC formation and complement-mediated immunity.

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