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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

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Related Experiment Video

Updated: May 17, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Competing endogenous RNA database.

Aaron L Sarver1, Subbaya Subramanian

  • 1Masonic Cancer Center.

Bioinformation
|October 12, 2012
PubMed
Summary
This summary is machine-generated.

Alternate messenger RNAs (mRNAs) regulate specific genes by sequestering microRNAs (miRNAs). This study identifies competing endogenous RNAs (ceRNAs) genome-wide, revealing a fundamental genetic regulatory mechanism with biological relevance.

Keywords:
MREceRDBceRNAscompeting endogenous RNAs databasedatabasemicroRNA response elements

Related Experiment Videos

Last Updated: May 17, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Messenger RNAs (mRNAs) can be regulated by microRNAs (miRNAs).
  • The availability of miRNAs can be modulated by their interactions with other mRNAs.
  • This reciprocal regulation forms the basis of competing endogenous RNA (ceRNA) interactions.

Purpose of the Study:

  • To introduce the concept of competing endogenous RNAs (ceRNAs) as a fundamental genetic regulatory mechanism.
  • To develop a method for predicting ceRNAs based on shared miRNA response elements.
  • To identify ceRNAs for specific mRNAs on a genome-wide scale.

Main Methods:

  • Predicting miRNA response elements (MREs) based on molecular interaction and evolutionary conservation.
  • Examining the co-occurrence of MREs across mRNAs genome-wide.
  • Utilizing the competing endogenous RNA database (ceRDB) for predictions.

Main Results:

  • A genome-wide prediction of ceRNAs was performed.
  • The study identified specific mRNAs predicted to act as ceRNAs.
  • Predicted ceRNAs for PTEN were compared with existing literature, showing biological relevance.

Conclusions:

  • mRNA-mRNA crosstalk via shared miRNA response elements is a key regulatory mechanism.
  • The developed method accurately predicts ceRNAs with biological significance.
  • The ceRDB provides a valuable resource for understanding ceRNA networks.