Related Experiment Video
Updated: May 17, 2026
06:18
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
Published on: August 13, 2019
Progesterone pharmacokinetics in the mouse: implications for potential stroke therapy
Raymond Wong1, David Ray, David A Kendall
1Division of Stroke, University of Nottingham, Clinical Sciences Building, City Hospital Campus School of Biomedical Sciences, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, UK. msxrw@nottingham.ac.uk
The Journal of Pharmacy and Pharmacology
|October 13, 2012
Summary
Continuous delivery of progesterone via osmotic minipump effectively increases brain concentrations compared to bolus injection. This method enhances progesterone
Area of Science:
- Neuroscience
- Pharmacology
- Biomedical Engineering
Background:
- Progesterone exhibits neuroprotective properties in preclinical models of central nervous system injury, including cerebral ischemia.
- Understanding progesterone's pharmacokinetic profile is crucial for optimizing its therapeutic potential.
Purpose of the Study:
- To investigate the impact of different progesterone dosing regimens on brain levels.
- To characterize the pharmacokinetic profile of progesterone delivery in a preclinical model.
Main Methods:
- Male C57 Bl/6 mice received intraperitoneal progesterone injections or continuous subcutaneous infusion via osmotic minipumps.
- Plasma and brain samples were collected over 24-48 hours.
- Progesterone concentrations were quantified using enzyme-linked immunoassay.
Main Results:
- Intraperitoneal progesterone demonstrated a short half-life in both plasma and brain.
- Minipump delivery resulted in sustained higher progesterone concentrations in plasma and brain.
- The volume of distribution was significantly greater with minipump delivery compared to intraperitoneal injection.
Conclusions:
- A loading dose followed by continuous minipump infusion is an effective strategy for delivering progesterone to the brain.
- This enhanced delivery method holds promise for neuroprotective therapies.
