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Updated: May 17, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Spectroscopic analysis and biodegradation potential study of dissolved organic matters in sewage sludge treated with
Yuxuan Zhang1, Panyue Zhang, Jianbin Guo
1Beijing Key Lab for Source Control Technology of Water Pollution, Beijing Forestry University, Beijing 100083, China. zzyyxx0618@163.com
Abstract:
The effect of high-pressure homogenization (HPH) treatment on characteristics of dissolved organic matters (DOM) in sewage sludge was investigated. Soluble chemical oxygen demand (SCOD), dissolved organic carbon (DOC), protein and polysaccharide concentration in sludge supernatant significantly increased after HPH treatment. Fourier-transform infrared (FTIR) spectra showed that the main components in the DOM for raw sludge were protein and polysaccharide, while for the treated sludge were protein, polysaccharide and lipid. The spectra of fluorescence excitation-emission matrix (EEM) for DOM showed two protein-like peaks at the excitation/emission wavelengths (Ex/Em) of 225/330-340 nm and 275/310-335 nm. A single broad shoulder representing substances with aromatic structure in range 245-270 nm was found in ultraviolet (UV) spectra of the DOM for the treated sludge. Homogenization pressure and homogenization cycle number affected the content of aromatic carbon per unit DOC. The maximum BOD5/SCOD ratio of 0.48 was achieved at 60 MPa with a single homogenization cycle.
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