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Phosphorylated dolichols in aging.

R K Pullarkat1, P S Pullarkat, G N Morris

  • 1New York State Office of Mental Retardation and Developmental Disabilities, Staten Island 10314.

The Biochemical Journal
|February 1, 1990
PubMed
Summary
This summary is machine-generated.

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Aging brains show increased dolichyl diphosphate oligosaccharide (Dol-PP-oligo) levels, suggesting reduced utilization for N-glycosylation. Liver synthesis rates increased, but levels remained stable, indicating active processing of these glycoprotein precursors.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Gerontology

Background:

  • Dolichyl phosphate and its derivatives are crucial for N-glycosylation, a vital post-translational modification.
  • Age-associated changes in these pathways may impact cellular function and contribute to aging phenotypes.

Purpose of the Study:

  • To investigate age-related alterations in dolichyl phosphate and dolichyl diphosphate derivative levels and synthesis in mouse brain and liver.
  • To elucidate the implications of these changes for glycoprotein synthesis and cellular metabolism during aging.

Main Methods:

  • Quantification of phosphorylated dolichols and dolichyl diphosphate oligosaccharides in brain, liver, and kidney tissues from mice of different ages.
  • Measurement of the incorporation of labeled glucose into dolichyl phosphate glucose and dolichyl diphosphate oligosaccharides in brain and liver microsomes.

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Main Results:

  • Phosphorylated dolichols and dolichyl diphosphate oligosaccharides significantly increased in the aging brain but remained unchanged in the liver and kidney.
  • While synthesis rates were unaffected by age in brain microsomes, liver microsomes showed a 50-150% increase in the synthesis of these components.
  • The accumulation of dolichyl diphosphate oligosaccharides in the aging brain suggests potential defects in utilization or catabolism.

Conclusions:

  • Aging impacts dolichol metabolism differently across tissues, with the brain exhibiting accumulation of key glycoprotein precursors.
  • The observed accumulation in the brain may indicate reduced N-glycosylation efficiency or impaired catabolism with age.
  • Increased synthesis in the liver, without accumulation, points to enhanced turnover or utilization of these molecules in aging liver tissue.