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Acidic and basic fibroblast growth factor mRNAs are expressed by skeletal muscle satellite cells
J Alterio1, Y Courtois, J Robelin
1Unité de Recherche Gérontologique, INSERM, Paris, France.
Abstract:
We postulated that Fibroblast Growth Factor (FGF) involved in fetal or regenerative morphogenesis of skeletal muscle originated from this tissue. Using a bovine retina cDNA probe encoding acidic FGF, we showed that growing muscles from bovine fetuses express this mRNA, but that this expression is reduced in neonate muscles. Cultures of proliferating satellite cells isolated from adult rat muscles expressed aFGF mRNA strongly but bFGF mRNA weakly; these mRNAs disappeared in cells differentiated into myotubes. 10(-7)M 12-O-tetradecanoyl phorbol -13-acetate (TPA) increased aFGF mRNA expression in both proliferating and differentiated satellite cells. Contrastingly, proliferating L6 myogenic cells only expressed aFGF mRNA significantly under TPA treatment. Therefore, the satellite cells did seem to be a possible source for FGF, especially aFGF, which might regulate the myogenic process.
Insights
Skeletal muscle satellite cells may produce acidic Fibroblast Growth Factor (aFGF), a key factor in muscle development and regeneration. This study found aFGF mRNA in satellite cells, suggesting its role in regulating the myogenic process.
Area of Science:
- Molecular Biology
- Cell Biology
- Developmental Biology
Background:
- Fibroblast Growth Factor (FGF) is implicated in skeletal muscle morphogenesis.
- The origin of FGF in fetal and regenerative muscle development remains unclear.
Purpose of the Study:
- To investigate if skeletal muscle satellite cells are a source of FGF.
- To determine the role of FGF, particularly acidic FGF (aFGF), in regulating the myogenic process.
Main Methods:
- RT-PCR analysis of aFGF and bFGF mRNA expression in bovine fetal and adult rat skeletal muscles and satellite cells.
- Culture of rat satellite cells and L6 myogenic cells.
- Treatment with 12-O-tetradecanoyl phorbol -13-acetate (TPA) to assess its effect on FGF mRNA expression.
Main Results:
- Growing bovine fetal muscles expressed aFGF mRNA, with reduced expression in neonate muscles.
- Proliferating adult rat satellite cells strongly expressed aFGF mRNA, which diminished upon differentiation into myotubes.
- TPA treatment upregulated aFGF mRNA in both proliferating and differentiated satellite cells and in L6 myogenic cells.
Conclusions:
- Skeletal muscle satellite cells are a potential source of FGF, especially aFGF.
- aFGF produced by satellite cells may play a regulatory role in the myogenic process.
- TPA influences aFGF expression in satellite cells and myogenic cells.