Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Protein Organization

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.

Protein Organization

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.

Protein Organization

Protein Organization

Protein Organization

Protein Organization

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting bacterial kinases as a strategy to counteract antibiotic resistance.

Communications chemistry·2025

Same author

Fragment-based drug design of a bacterial kinase inhibitor capable of increasing the antibiotic sensitivity of clinical isolates.

Communications chemistry·2025

Same author

Identification and characterization of a nonpeptidic cyclophilin ligand with antiviral activity against feline and porcine α-coronaviruses.

Veterinary research·2025

Same author

Picomolar bivalent inhibitors of protein kinase CK2 active against β-coronavirus replication.

European journal of medicinal chemistry·2025

Same author

Cryo-EM structure and regulation of human NAD kinase.

Science advances·2025

Same author

Binding-Site Switch for Protein Kinase CK2 Inhibitors.

ChemMedChem·2025

Search research articles

Related Experiment Video

Updated: May 17, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

Fragment and conquer: from structure to complexes to function.

Jean-François Guichou¹, Gilles Labesse

¹Atelier de Bio- et Chimie Informatique Structurale, CNRS, UMR5048, Centre de Biochimie Structurale, F-34090 Montpellier, France.

Structure (London, England : 1993)

|October 16, 2012

Summary

This summary is machine-generated.

Researchers used X-ray crystallography metabolite screening to connect an unknown protein domain to an unknown biochemical function. This breakthrough enables large-scale functional annotation and advances structural genomics.

More Related Videos

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

Related Experiment Videos

Last Updated: May 17, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

Area of Science:

Structural biology
Biochemistry
Genomics

Background:

Orphan protein domains and biochemical functions present challenges in biological research.
Identifying the functions of these 'orphan' entities is crucial for understanding cellular processes.

Discussion:

Shumilin and colleagues demonstrate a powerful method combining X-ray crystallography and metabolite screening.
This approach successfully links a previously uncharacterized protein domain with a specific biochemical activity.

Key Insights:

Metabolite screening via X-ray crystallography is an effective strategy for functional annotation.
The study establishes a novel link between an orphan domain and an orphan function.
This work highlights the utility of structural biology techniques in uncovering biochemical roles.

Outlook:

This methodology can be applied to large-scale functional annotation of the proteome.
It sets new directions and objectives for the field of structural genomics.
Future research can leverage this technique to accelerate the discovery of protein functions.