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Updated: May 17, 2026

Generation of Alginate Microspheres for Biomedical Applications
Published on: August 12, 2012
Development and in vitro characterization of insulin loaded whey protein and alginate microparticles
Emmanuelle Déat-Lainé1, Valérie Hoffart, Jean-Michel Cardot
1Université d'Auvergne, UFR Pharmacie, Equipe de Recherche Technologique Conception, Ingénierie et Développement de l'Aliment et du Médicament (ERT CIDAM), Clermont-Ferrand F-63001, France.
Abstract:
Insulin was encapsulated into microparticles (MP) made of denaturized whey proteins (WP) and alginate (ALG) using an extrusion/cold gelation process with calcium ions. High encapsulation efficiency of 85% was obtained. Influence of insulin on polymeric viscosity and on microparticle behavior was evaluated. Insulin seemed to interact with WP chains by non covalent binding and steric hindrance. This influence was balanced by ALG addition. Nevertheless, insulin was released rapidly by diffusion at both acidic and intestinal dissolution media. Despite this fast in vitro release, WP/ALG MP showed an important enzymatic inhibition effect on trypsin and alpha-chymotrypsin. Thus, WP/ALG MP contributed to an effective insulin protection towards enzymatic degradation. The aforementioned results suggested that WP based microparticles are a promising carrier for improving oral delivery of insulin.
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