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Matrix metalloproteinases and their impact on sinusal extension in chronic rhinosinusitis with nasal polyps
Rafael Rossell Malinsky1, Fabiana C P Valera, Fransérgio Emílio Cavallari
1Department of Ophthalmology, Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brazil.
Unlabelled:
MMPs (metalloproteinases) and their inhibitor TIMP (tissue inhibitor of metalloproteinases) are important in tissue remodeling and have been implicated in nasal polyp pathophysiology. The objective of the present study was to evaluate gene expression of MMP-1, MMP-2, MMP-9 and TIMP-1 gene expression in nasal polyps and compare them with normal nasal mucosa. Since MMPs could induce a more prominent tissue edema, we also assessed if these gene expressions could be related to a more extensive disease. For the experimental study, 30 nasal polyp samples from patients with CRSwNP (Chronic Rhinosinusitis with Nasal Polyposis) and 19 middle turbinates (MT) from controls were obtained. Gene expression of MMP-1, MMP-2, MMP-9 and TIMP-1 was assessed by qRT-PCR. The expressions of these genes were compared between nasal polyps and controls and correlated to each individual Lund-Mackay score, MMP-1, MMP-2 and MMP-9 were significantly more expressed in nasal polyps than in controls (P < 0.005, P < 0.0001 and P < 0.05 respectively). No expression of TIMP-1 mRNA was detected. There was no correlation between disease extension evaluated by the Lund-Mackay score and MMPs gene expression. The present results demonstrate that there is a significantly higher mRNA gene expression of MMP-1, MMP-2 and MMP-9 in patients with CRSwNP than controls. However, no correlation was observed between MMPs gene expression and sinonasal CT scan extension.
Level Of Evidence:
2c.
Insights
Gene expression of MMP-1, MMP-2, and MMP-9 is significantly higher in nasal polyps compared to healthy tissue. However, this increased expression does not correlate with disease severity in Chronic Rhinosinusitis with Nasal Polyposis.
Area of Science:
- Biomedical research
- Molecular biology
- Otorhinolaryngology
Background:
- Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play roles in tissue remodeling.
- These molecules are implicated in the pathophysiology of nasal polyps.
Purpose of the Study:
- To evaluate gene expression of MMP-1, MMP-2, MMP-9, and TIMP-1 in nasal polyps.
- To compare gene expression between nasal polyps and normal nasal mucosa.
- To assess the relationship between MMP gene expression and disease extent in Chronic Rhinosinusitis with Nasal Polyposis (CRSwNP).
Main Methods:
- Quantitative real-time PCR (qRT-PCR) was used to assess gene expression.
- Samples were obtained from 30 CRSwNP patients and 19 controls (middle turbinates).
- Gene expression levels were compared between groups and correlated with Lund-Mackay scores.
Main Results:
- MMP-1, MMP-2, and MMP-9 mRNA expression were significantly higher in nasal polyps compared to controls.
- No TIMP-1 mRNA expression was detected in either group.
- No correlation was found between MMP gene expression and disease extension (Lund-Mackay score).
Conclusions:
- Patients with CRSwNP exhibit significantly elevated mRNA expression of MMP-1, MMP-2, and MMP-9.
- MMP gene expression levels do not correlate with the extent of sinonasal disease as assessed by CT scans.
