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Updated: May 17, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Elucidating the regulation of T cell subsets (review)
Yasuko Kitagishi1, Mayumi Kobayashi, Yurie Yamashina
1Department of Environmental Health Science, Nara Women's University, Kita-Uoya Nishimachi, Nara 630-8506, Japan.
Abstract:
CD4-positive T lymphocytes mainly direct immune as well as autoimmune responses against a variety of pathogens or allergens. This is achieved through the acquisition of specialized functions followed by differentiation into various T cell subsets. The differentiation process of naive T cells into effector subsets is regulated by dendritic cells and secreted cytokines. Signal transducer and activator of transcription proteins play critical roles in transmitting cytokine-mediated signals and specifying T cell differentiation. Epigenetic changes such as histone acetylation and methylation along with DNA methylation also regulate expression of differentiation-specific genes. Defining exactly how extrinsic signals control the specification of T cells will provide important insights and therapeutic opportunities.
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