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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: May 17, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

A single run LC-MS/MS method for phospholipidomics.

Corinne Buré1, Sophie Ayciriex, Eric Testet

  • 1Chimie Biologie des Membranes et Nanoobjets CBMN-UMR 5248 Centre de Génomique Fonctionnelle Université Bordeaux 2, Université de Bordeaux, 146, rue Léo Saignat, 33076 Bordeaux cedex, France. c.bure@cbmn.u-bordeaux.fr

Analytical and Bioanalytical Chemistry
|October 16, 2012
PubMed
Summary
This summary is machine-generated.

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers a superior single-run analysis for whole cell phospholipids compared to shotgun analysis. Reversed-phase HPLC-MS/MS is recommended for its enhanced selectivity and repeatability in phospholipidome studies.

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Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Related Experiment Videos

Last Updated: May 17, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Area of Science:

  • Lipidomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Phospholipids are crucial cellular components involved in various biological processes.
  • Accurate and efficient analysis of phospholipid profiles is essential for understanding cellular function and disease.
  • Existing methods like shotgun analysis have limitations in comprehensive phospholipidome profiling.

Purpose of the Study:

  • To compare liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with shotgun analysis for single-run whole cell phospholipid analysis.
  • To evaluate different LC modes (HILIC, NP, RP) for phospholipid profiling.
  • To identify the optimal LC-MS/MS method for robust and comprehensive phospholipidome analysis.

Main Methods:

  • Evaluation of hydrophilic interaction liquid chromatography (HILIC), normal phase (NP), and reversed phase (RP) liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
  • Analysis of yeast phospholipidome using wild-type and acyltransferase-deficient strains.
  • Utilized multiple reaction monitoring (MRM) mode for relative quantitation of phospholipids.
  • Compared LC-MS/MS performance against shotgun analysis.

Main Results:

  • Combined LC-MS/MS methods identified a greater number of quantified phospholipid species than shotgun analysis.
  • Normal phase (NP) and reversed phase (RP) HPLC-ESI-MS/MS were applied to yeast phospholipidome.
  • Reversed-phase HPLC-MS/MS demonstrated superior selectivity, robustness, and repeatability.
  • MRM mode enabled relative quantitation using reference phospholipids with odd-numbered fatty acid chains.

Conclusions:

  • LC-MS/MS, particularly RP-HPLC-MS/MS, is superior to shotgun analysis for comprehensive single-run phospholipidome analysis.
  • RP-HPLC-MS/MS offers improved selectivity, robustness, and repeatability for phospholipid profiling.
  • This optimized LC-MS/MS approach enhances the ability to quantify diverse phospholipid species in cellular samples.