Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-neutralization of SARS-CoV-2 BA.3.2.2 lineage by JN.1 mRNA vaccine-induced immunity.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

[Pathophysiology of Influenza Encephalopathy].

Brain and nerve = Shinkei kenkyu no shinpo·2026
Same author

Alarmin cues and regulated cell death calibrate mucosal immunoglobulin A responses and virologic control after intranasal whole-virion inactivated influenza vaccination.

International immunology·2026
Same author

Immunogenicity of influenza split vaccine for 2025/26 season in Japan using a mouse model.

Japanese journal of infectious diseases·2026
Same author

Applications of Pseudoviruses Bearing Glycoproteins of SARS-CoV-2 and Influenza Virus.

Advances in experimental medicine and biology·2026
Same author

Potent efficacy of an NA-targeting antibody against a broad spectrum of H5N1 influenza viruses.

Nature communications·2026

Related Experiment Video

Updated: May 17, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

Spi-B is critical for plasmacytoid dendritic cell function and development.

Izumi Sasaki1, Katsuaki Hoshino, Takahiro Sugiyama

  • 1Laboratory for Host Defense, RIKEN Research Center for Allergy and Immunology, Kanagawa, Japan.

Blood
|October 16, 2012
PubMed
Summary

Spi-B is crucial for plasmacytoid dendritic cell (pDC) development and function. Spi-B deficiency impairs type I interferon production and alters pDC distribution, highlighting its essential role in immune responses.

More Related Videos

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
13:34

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

Related Experiment Videos

Last Updated: May 17, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
13:34

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Plasmacytoid dendritic cells (pDCs) are key immune cells producing type I interferons (IFNs) via Toll-like receptors (TLRs) 7 and 9.
  • The molecular mechanisms governing pDC development and function are not fully understood.

Purpose of the Study:

  • To investigate the role of the Ets family transcription factor Spi-B in pDC function and development.
  • To elucidate the molecular mechanisms by which Spi-B influences type I IFN production.

Main Methods:

  • Analysis of Spi-B expression in pDCs.
  • Studies using Spi-B-deficient pDCs and mice.
  • Assessment of type I IFN production and gene expression.
  • Evaluation of pDC development and distribution.

Main Results:

  • Spi-B cooperates with IRF-7 to activate type I IFN promoters.
  • Spi-B deficiency leads to impaired TLR7/9-induced type I IFN production.
  • Spi-B-deficient mice exhibit decreased bone marrow pDCs and increased peripheral pDCs.
  • Spi-B is involved in pDC development, with defects being intrinsic to pDCs.

Conclusions:

  • Spi-B plays critical roles in both the function and development of plasmacytoid dendritic cells.
  • Spi-B is essential for proper type I IFN production and pDC homeostasis.
  • Understanding Spi-B's role provides insights into pDC biology and immune regulation.