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Related Concept Videos

Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
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Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Related Experiment Video

Updated: May 17, 2026

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

NMR-based diffusion pore imaging by double wave vector measurements.

Tristan Anselm Kuder1, Frederik Bernd Laun

  • 1Department of Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Magnetic Resonance in Medicine
|October 16, 2012
PubMed
Summary
This summary is machine-generated.

Nuclear magnetic resonance (NMR) diffusion experiments can now determine the shape of closed pores using short gradient pulses. This new method overcomes limitations of previous techniques, offering faster convergence and flexible NMR sequence design.

Keywords:
MRIdiffusionpore shapeporous mediaq‐space imagingrestrictions

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Related Experiment Videos

Last Updated: May 17, 2026

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
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From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

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Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
12:19

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Published on: May 27, 2012

Area of Science:

  • Physics
  • Chemistry
  • Materials Science

Background:

  • Nuclear magnetic resonance (NMR) diffusion experiments investigate boundaries like cell membranes.
  • NMR diffusion measurements collect signals from the entire sample, avoiding signal loss at higher resolutions.
  • Determining the exact shape of closed pores using NMR diffusion has been a long-standing challenge.

Purpose of the Study:

  • To present a novel method for determining the shape of arbitrary closed pores using NMR diffusion measurements.
  • To overcome limitations of previous approaches in pore shape determination.

Main Methods:

  • Utilizing short diffusion gradient pulses exclusively.
  • Employing a method that does not require a priori knowledge of pore shape.
  • Achieving faster convergence to the diffusion long-time limit.

Main Results:

  • The developed method successfully reveals the shape of arbitrary closed pores.
  • Reduced demands on relaxation times compared to former approaches.
  • Enabled more flexible NMR sequence design, including the use of stimulated echoes.

Conclusions:

  • The presented NMR diffusion method enables the determination of closed pore shapes without prior assumptions.
  • This technique offers advantages in terms of speed and flexibility for diffusion NMR studies.