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Sustained, localized transgene expression mediated from lentivirus-loaded biodegradable polyester elastomers.

Michele C Jen1, Kevin Baler, Ashleigh R Hood

  • 1Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, USA.

Journal of Biomedical Materials Research. Part A
|October 16, 2012
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Summary

Biodegradable elastomers Poly(1,8-octanediol citrate) (POC) and poly(glycerol-sebacate) (PGS) can immobilize lentivirus for sustained gene delivery. This biomaterial approach shows promise for tissue engineering and regenerative medicine applications.

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Area of Science:

  • Biomaterials science
  • Gene therapy
  • Tissue engineering
  • Regenerative medicine

Background:

  • Gene delivery is crucial for tissue engineering and regenerative medicine.
  • Biodegradable, biocompatible elastomers like POC and PGS offer tunable properties.
  • Investigating new biomaterials and vectors is essential for advancing these fields.

Purpose of the Study:

  • To assess the feasibility of using POC and PGS for lentivirus immobilization.
  • To evaluate sustained and localized transgene expression from immobilized lentivirus.
  • To determine the potential of these biomaterials in tissue engineering and regenerative medicine.

Main Methods:

  • Preparation of porous POC and PGS biomaterials using salt as a porogen.
  • In vitro assessment of transgene expression in cells seeded onto biomaterials.
  • In vivo subcutaneous implantation of biomaterials with immobilized lentivirus in rats.
  • Evaluation of lentivirus activity and transgene expression over time.

Main Results:

  • POC and PGS effectively supported lentivirus immobilization and release.
  • Titer-dependent transgene expression was observed in vitro.
  • Lentivirus activity was maintained on biomaterials for at least 5 days in vitro.
  • Sustained and localized transgene expression was achieved in vivo for at least 5 weeks.

Conclusions:

  • POC and PGS are suitable biomaterials for lentivirus immobilization.
  • This method enables sustained and localized transgene expression.
  • Immobilized lentivirus on POC and PGS holds significant potential for tissue engineering and regenerative medicine.