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Updated: May 17, 2026
Oral Biofilm Formation on Different Materials for Dental Implants
Published on: June 24, 2018
Evaluation of bacterial adhesion on machined titanium, Osseotite® and Nanotite® discs
Ruggero Rodriguez y Baena1, Carla Renata Arciola, Laura Selan
1Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, University of Pavia, Pavia, Italy.
Purpose:
Bacterial adhesion and colonization play a crucial function in the pathogenesis of peri-implant tissue infection, which is considered the main cause of fixture loss. The aim of this study is to evaluate the differences in bacterial adhesion between a machined titanium surface, a double acid etched surface (Osseotite®) and an Osseotite surface with Nanometer-scale Discrete Crystalline Deposition (DCD™) of calcium phosphate (CaP)(Nanotite®).
Methods:
Surface roughness properties of each sample were determined by a laser profilometer and scanning electron microscopy (SEM) observation. Bacterial adhesion on machined, Osseotite®, and Nanotite® discs were performed using the following bacterial strains: Streptococcus mutans CCUG 35176, Streptococcus sanguis CCUG 17826, Streptococcus salivarius CCUG 11878, Actinobacillus actinomycetecomitans CCUG 37002, Porphyromonas gingivalis CCUG 2521. The assessment of bacterial adhesion was performed by comparing two methods: Total Viable Count (TVC) estimation and Confocal Laser Scanning Microscopic (CSLM) studies.
Results:
The surface roughness parameter increased as follows: machined
Conclusions:
Nanotite® samples showed the lowest amount of bacterial contamination in comparison to the smoother machined and rougher Osseotite® surfaces.
