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Updated: May 17, 2026

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
Published on: April 21, 2023
Fast DNA interstrand cross-linking reaction by 6-vinylpurine
Shuhei Imoto1, Tomoko Chikuni, Hisao Kansui
1Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto-city, Kumamoto, Japan. simoto@ph.sojo-u.ac.jp
Abstract:
Oligonucleotides that incorporate a reactive moiety to form an interstrand cross-link have been widely studied for their potential toward inhibiting gene expression or as basic tools for chemical biology studies. The 6-vinylpurine (2) newly designed in the current study serves well as a new purine-base moiety for increasing cross-link reactivity to target cytosine. Thus, oligonucleotides containing 6-vinylpurine exhibit a more selective and much smoother DNA cross-linking ability to cytosine than the oligonucleotide analogs derived from 2-amino-6-vinylpurine (1) previously explored.
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