Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Clonal chromosomal changes in chronic lymphocytic leukemia.

B N Nayak¹, J Sokal, M Ray

¹Department of Pediatrics, University of Manitoba, Faculty of Medicine, Winnipeg, Canada.

|February 1, 1990

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Para-aminopropiophenone toxicity in domestic dogs: a description of non-target toxicosis in Victoria, Australia: 13 dogs (2016-2023).

Australian veterinary journal·2025

Same author

Fragment-based QSAR study to explore the structural requirements of DPP-4 inhibitors: a stepping stone towards better type 2 diabetes mellitus management.

SAR and QSAR in environmental research·2024

Same author

Women's cardiovascular health - the cardio-oncologic jigsaw.

Climacteric : the journal of the International Menopause Society·2023

Same author

Learning Curve of Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy - an Analysis of Critical Perioperative and Surgical Outcomes among 155 Peritoneal Surface Malignancy Patients Treated at a Tertiary Care Cancer Centre.

Clinical oncology (Royal College of Radiologists (Great Britain))·2022

Same author

In their own words: investigating the preparedness of final year dental students in the UK for independent general dental practice.

British dental journal·2018

Same author

Bifidobacteria and its rice fermented products on diet induced obese mice: analysis of physical status, serum profile and gene expressions.

Beneficial microbes·2018

Cytogenetic analysis in 28 B-cell chronic lymphocytic leukemia (B-CLL) patients revealed frequent clonal chromosome aberrations. Abnormalities were observed in 50% of patients, with double minute chromosomes found in drug-resistant cases.

Area of Science:

Hematology
Cytogenetics
Oncology

Background:

B-cell chronic lymphocytic leukemia (B-CLL) is a heterogeneous lymphoproliferative disorder.
Understanding the cytogenetic landscape of B-CLL is crucial for prognosis and treatment.
In vitro culture methods are used to assess lymphocyte responsiveness and chromosomal abnormalities.

Purpose of the Study:

To perform cytogenetic analysis on B-CLL patients.
To investigate the in vitro growth and chromosomal response of B-lymphocytes.
To identify specific chromosomal aberrations associated with B-CLL and drug resistance.

Main Methods:

Cytogenetic analysis of peripheral blood or isolated B-lymphocytes from 28 B-CLL patients.
In vitro incubation of cells for 5-7 days, stimulated by pokeweed mitogen (PWM) or phorbol myristate-acetate (PMA).

Related Experiment Videos

Addition of conditioned medium (CM) from T-cell leukemia or B-cell growth factor (BCGF) to assess cellular response and mitotic index.

Main Results:

Abnormal karyotypes were found in 22 out of 28 patients (79%).
Clonal chromosome aberrations, including numerical and structural changes, were identified in 14 patients (50%).
Double minute chromosomes (dmin) were observed in 4 drug-resistant patients, indicating potential mechanisms of resistance.

Conclusions:

Cytogenetic analysis is a valuable tool for characterizing B-CLL.
Specific clonal chromosome aberrations are prevalent in B-CLL patients.
The presence of double minute chromosomes may be linked to resistance to anti-neoplastic drugs in B-CLL.