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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Light-induced cell detachment for cell sheet technology.

Yi Hong¹, Mengfei Yu, Wenjian Weng

¹Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.

|October 17, 2012

Summary

This summary is machine-generated.

Researchers developed a novel light-induced cell detachment method using titanium dioxide (TiO2) nanodots and UV light. This technique rapidly harvests intact, viable cell sheets for further applications.

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A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Area of Science:

Biomaterials Science
Cell Biology
Tissue Engineering

Background:

Cell harvesting is crucial for tissue engineering and research.
Current methods can be time-consuming and may damage cells.
Developing non-invasive, efficient cell detachment techniques is essential.

Purpose of the Study:

To investigate the light-induced detachment of cells from a TiO2 nanodot-coated surface.
To evaluate the viability and applicability of detached cells and cell sheets.
To assess the biocompatibility and safety of the proposed method.

Main Methods:

Culturing mouse calvaria-derived pre-osteoblastic cells (MC3T3-E1) on TiO2 nanodot-coated quartz substrates.
Exposing cell cultures to UV365 illumination for 20 minutes.
Analyzing cell detachment rates, cell viability, and the integrity of harvested cell sheets.
Conducting biocompatibility and safety assessments.

Main Results:

Over 90% of MC3T3-E1 cells detached within 20 minutes of UV365 illumination.
Intact cell sheets were successfully harvested.
Detached cells maintained high viability and were suitable for subsequent culture and applications.
TiO2 nanodots and UV365 illumination demonstrated safety and biocompatibility.

Conclusions:

Light-induced cell detachment using TiO2 nanodots offers a rapid and efficient method for cell and cell sheet harvesting.
The method preserves cell viability and integrity, making it suitable for various downstream applications.
Adsorbed extracellular matrix proteins are important for cell sheet development and biocompatibility in this system.