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Updated: May 17, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Orchid fleck virus: an unclassified bipartite, negative-sense RNA plant virus
De Wei Peng1, Guo Hua Zheng, Zhi Zhong Zheng
1Xiamen Overseas Chinese Subtropical Plant Introduction Garden, National Plant Introduction Quarantine Base, Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen, 361002, Fujian, China.
Abstract:
Orchid fleck virus (OFV) causes chlorotic or necrotic spots in many orchid species. Its particle morphology and cytopathic effects are similar to those of nucleorhabdoviruses. Although OFV shares clear sequence similarities with rhabdoviruses, its taxonomic status is undetermined because its negative-sense RNA genome is bipartite. This review presents a general overview of classical and contemporary findings about etiology, serology, epidemiology, pathology, molecular biology, detection and prevention methods of orchid fleck virus. Because of the characteristics of OFV and viruses of the Rhabdoviridae and Mononegavirales, it is proposed that a new genus of negative-sense RNA plant viruses outside of the Mononegavirales be established with orchid fleck virus as the type species.
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